diff --git a/lib/internal/test_runner/runner.js b/lib/internal/test_runner/runner.js
index 9590ef8dcf7..e2237dd73f4 100644
--- a/lib/internal/test_runner/runner.js
+++ b/lib/internal/test_runner/runner.js
@@ -34,6 +34,7 @@ const { spawn } = require('child_process');
 const { finished } = require('internal/streams/end-of-stream');
 const { resolve } = require('path');
 const { DefaultDeserializer, DefaultSerializer } = require('v8');
+const { getOptionValue } = require('internal/options');
 const { Interface } = require('internal/readline/interface');
 const { deserializeError } = require('internal/error_serdes');
 const { Buffer } = require('buffer');
@@ -697,6 +698,11 @@ function run(options = kEmptyObject) {
 
           root.harness.bootstrapPromise = promise;
 
+          const userImports = getOptionValue('--import');
+          for (let i = 0; i < userImports.length; i++) {
+            await cascadedLoader.import(userImports[i], parentURL, kEmptyObject);
+          }
+
           for (let i = 0; i < testFiles.length; ++i) {
             const testFile = testFiles[i];
             const fileURL = pathToFileURL(testFile);
diff --git a/test/fixtures/test-runner/no-isolation/global-hooks.js b/test/fixtures/test-runner/no-isolation/global-hooks.js
new file mode 100644
index 00000000000..cc8fab98603
--- /dev/null
+++ b/test/fixtures/test-runner/no-isolation/global-hooks.js
@@ -0,0 +1,6 @@
+import('node:test').then((test) => {
+  test.before(() => console.log('before(): global'));
+  test.beforeEach(() => console.log('beforeEach(): global'));
+  test.after(() => console.log('after(): global'));
+  test.afterEach(() => console.log('afterEach(): global'));
+});
diff --git a/test/fixtures/test-runner/no-isolation/one.test.js b/test/fixtures/test-runner/no-isolation/one.test.js
index 69e0485a371..5b5cc2b025c 100644
--- a/test/fixtures/test-runner/no-isolation/one.test.js
+++ b/test/fixtures/test-runner/no-isolation/one.test.js
@@ -3,30 +3,35 @@ const { before, beforeEach, after, afterEach, test, suite } = require('node:test
 
 globalThis.GLOBAL_ORDER = [];
 
+function record(data) {
+  globalThis.GLOBAL_ORDER.push(data);
+  console.log(data);
+}
+
 before(function() {
-  GLOBAL_ORDER.push(`before one: ${this.name}`);
+  record(`before one: ${this.name}`);
 });
 
 beforeEach(function() {
-  GLOBAL_ORDER.push(`beforeEach one: ${this.name}`);
+  record(`beforeEach one: ${this.name}`);
 });
 
 after(function() {
-  GLOBAL_ORDER.push(`after one: ${this.name}`);
+  record(`after one: ${this.name}`);
 });
 
 afterEach(function() {
-  GLOBAL_ORDER.push(`afterEach one: ${this.name}`);
+  record(`afterEach one: ${this.name}`);
 });
 
 suite('suite one', function() {
-  GLOBAL_ORDER.push(this.name);
+  record(this.name);
 
   test('suite one - test', { only: true }, function() {
-    GLOBAL_ORDER.push(this.name);
+    record(this.name);
   });
 });
 
 test('test one', function() {
-  GLOBAL_ORDER.push(this.name);
+  record(this.name);
 });
diff --git a/test/fixtures/test-runner/no-isolation/two.test.js b/test/fixtures/test-runner/no-isolation/two.test.js
index 50ae6541ce1..4fbc7dfc840 100644
--- a/test/fixtures/test-runner/no-isolation/two.test.js
+++ b/test/fixtures/test-runner/no-isolation/two.test.js
@@ -1,30 +1,35 @@
 'use strict';
 const { before, beforeEach, after, afterEach, test, suite } = require('node:test');
 
+function record(data) {
+  globalThis.GLOBAL_ORDER.push(data);
+  console.log(data);
+}
+
 before(function() {
-  GLOBAL_ORDER.push(`before two: ${this.name}`);
+  record(`before two: ${this.name}`);
 });
 
 beforeEach(function() {
-  GLOBAL_ORDER.push(`beforeEach two: ${this.name}`);
+  record(`beforeEach two: ${this.name}`);
 });
 
 after(function() {
-  GLOBAL_ORDER.push(`after two: ${this.name}`);
+  record(`after two: ${this.name}`);
 });
 
 afterEach(function() {
-  GLOBAL_ORDER.push(`afterEach two: ${this.name}`);
+  record(`afterEach two: ${this.name}`);
 });
 
 suite('suite two', function() {
-  GLOBAL_ORDER.push(this.name);
+  record(this.name);
 
   before(function() {
-    GLOBAL_ORDER.push(`before suite two: ${this.name}`);
+    record(`before suite two: ${this.name}`);
   });
 
   test('suite two - test', { only: true }, function() {
-    GLOBAL_ORDER.push(this.name);
+    record(this.name);
   });
 });
diff --git a/test/parallel/test-runner-no-isolation-hooks.mjs b/test/parallel/test-runner-no-isolation-hooks.mjs
new file mode 100644
index 00000000000..2c1570d2d75
--- /dev/null
+++ b/test/parallel/test-runner-no-isolation-hooks.mjs
@@ -0,0 +1,75 @@
+import * as common from '../common/index.mjs';
+import * as fixtures from '../common/fixtures.mjs';
+import { test } from 'node:test';
+
+const testArguments = [
+  '--test',
+  '--experimental-test-isolation=none',
+];
+
+const testFiles = [
+  fixtures.path('test-runner', 'no-isolation', 'one.test.js'),
+  fixtures.path('test-runner', 'no-isolation', 'two.test.js'),
+];
+
+const order = [
+  'before(): global',
+
+  'before one: <root>',
+  'suite one',
+
+  'before two: <root>',
+  'suite two',
+
+  'beforeEach(): global',
+  'beforeEach one: suite one - test',
+  'beforeEach two: suite one - test',
+
+  'suite one - test',
+  'afterEach(): global',
+  'afterEach one: suite one - test',
+  'afterEach two: suite one - test',
+
+  'beforeEach(): global',
+  'beforeEach one: test one',
+  'beforeEach two: test one',
+  'test one',
+
+  'afterEach(): global',
+  'afterEach one: test one',
+  'afterEach two: test one',
+
+  'before suite two: suite two',
+  'beforeEach(): global',
+  'beforeEach one: suite two - test',
+  'beforeEach two: suite two - test',
+
+  'suite two - test',
+  'afterEach(): global',
+  'afterEach one: suite two - test',
+  'afterEach two: suite two - test',
+
+  'after(): global',
+  'after one: <root>',
+  'after two: <root>',
+];
+
+test('Using --require to define global hooks works', async (t) => {
+  const spawned = await common.spawnPromisified(process.execPath, [
+    ...testArguments,
+    '--require', fixtures.path('test-runner', 'no-isolation', 'global-hooks.js'),
+    ...testFiles,
+  ]);
+
+  t.assert.ok(spawned.stdout.includes(order.join('\n')));
+});
+
+test('Using --import to define global hooks works', async (t) => {
+  const spawned = await common.spawnPromisified(process.execPath, [
+    ...testArguments,
+    '--import', fixtures.fileURL('test-runner', 'no-isolation', 'global-hooks.js'),
+    ...testFiles,
+  ]);
+
+  t.assert.ok(spawned.stdout.includes(order.join('\n')));
+});